Overview and Programme
The Structural Biology Cross Programme Acitivity "Structural Biology" together with Centre for Structural System Biology organizes a training course on methods and application in integrative structural biology. The main focus of this course is on sample preparation for biophysical studies, X-ray crystallography and cryo-electron microscopy including hands-on practical training. Lectures and keynote presentations will illustrate state-of-the-art examples for the combination of multiple structural biology techniques to study the structure and dynamics of biologically important and challenging systems.
Monday, 22 October
| Topic: Membranes | |
|---|---|
| Time: | Speakers: |
| 09:00 | Welcome Organisers |
| 09:15 | CSSB & DESY Michael Kolbe and Joerg Labahn |
| 09:45 | Protein Production Joop van den Heuvel |
| 10:30 | Coffee Break |
| 10:45 | Protein Characterization Maria Garcia Alai |
| 11:30 | Introduction to Pratical Course Ge Yang |
| 12:15 | Lunch |
| 13:15 | Pratical Course |
| Crystallization |
|
| Crystal Imaging |
|
| Crystal Harvesting |
|
| Spectroscopy |
|
| 18:30 | Dinner/ Poster Session |
Tuesday, 23 October
| Topic: Crystallography | |
|---|---|
| Time: | Speakers: |
| 09:00 | Presentation Wulf Blankenfeldt |
| 09:45 | Time Resolved Studies Arwen Pearson |
| 10:30 | Coffee Break |
| 10:45 | Serial Crystallography Thomas Schneider |
| 11:30 | Presentation tbd |
| 12:15 | Lunch |
| 13:15 | Pratical Course |
| 18:30 | Dinner/ Poster Session |
Wednesday, 24 October
| Topic: Complementary Methods | |
|---|---|
| Time: | Speakers: |
| 09:00 | Using NMR to study dynamics and regulatory interactions in integrative structural biology Michael Sattler |
| 09:45 | Solid state NMR Henrike Heise |
| 10:30 | Coffee Break |
| 10:45 | Integrative modeling of macromolecular assemblies Jan Kosinski |
11:30 | X-ray nanoscience at DESY Christian Schroer |
12:15 | Lunch |
| 13:15 | Native MS Alan Kadek |
15:00 | Excursion in Hamburg |
18:30 | Dinner at Restaurant |
Thursday, 25 October
| Topic: EM-Tomography | |
|---|---|
| Time: | Speakers: |
| 09:00 | Cryo Tomography Intro and Sub-Cellular Applications Kay Grünewald |
| 09:45 | Mechanistic insights from higher-order autophagy assemblies by cryo-EM Carsten Sachse |
| 10:30 | Coffee Break |
| 10:45 | Presentation Holger Stark |
| 11:30 | Super-Resolution Fluorescence Rainer Kaufmann |
| 12:15 | Lunch |
| 13:15 | Tomography Processing Practical Course |
| 18:30 | Dinner |
Friday, 26 October
| Topic: EM Single Particle | |
|---|---|
| Time: | Speakers: |
| 09:00 | Fibril Structure of Abeta(1-42) by Cryo-EM Gunnar Schroeder |
| 09:45 | Single Particle Analysis and Applications Jiri Wald |
| 10:30 | Coffee Break |
| 10:45 | Pratical Course Part I |
| 12:15 | Lunch |
| 13:15 | Pratical Course Part II |